Reflected light microscopy: Difference between revisions
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In reflected light microscope the light incident on the sample and is reflected so speculate. There is one half-mirror in which '''50% of the light is reflected''' and '''50% of the light is transmitid''', with losses in intensity image, but one gains the final resolution. | In reflected light microscope the light incident on the sample and is reflected so speculate. There is one half-mirror in which '''50% of the light is reflected''' and '''50% of the light is transmitid''', with losses in intensity image, but one gains the final resolution. | ||
Polarizing microscopy can be used with reflected and transmitted light. '''Reflected light is useful for study of opaque materials''' such as mineral oxides and sulfides, metals and silicon wafers and requires stress-free objectives that have not been corrected for viewing through a coverslip. | Polarizing microscopy can be used with reflected and transmitted light. '''Reflected light is useful for study of opaque materials''' such as mineral oxides and sulfides, metals and silicon wafers and requires stress-free objectives that have not been corrected for viewing through a coverslip. | ||
The reflected light microscope was created because it is not possible to observe metals with a transmitted light microscope, because the electrons of the conductive layer of metals interact strongly with photons, making these samples too little transparent. | The reflected light microscope was created because it is not possible to observe metals with a transmitted light microscope, because the electrons of the conductive layer of metals interact strongly with photons, making these samples too little transparent. | ||
Reflected light microscopy is often referred to as incident light, dpi-illumination, or metallurgical microscopy, and is the method of choice for fluorescence and for imaging specimens that remain opaque even when ground to a thickness of 30 micrometers. much like the fluorescense microscope, in reflected bright field microscopy the sample is illuminated from above through the objective. | Reflected light microscopy is often referred to as incident light, dpi-illumination, or metallurgical microscopy, and is the method of choice for fluorescence and for imaging specimens that remain opaque even when ground to a thickness of 30 micrometers. much like the fluorescense microscope, in reflected bright field microscopy the sample is illuminated from above through the objective. | ||
Reflected light microscopy is frequently the domain of industrial applications, especially in the rapidly growing semiconductor arena and this represents a most important segment of microscopical studies. | Reflected light microscopy is frequently the domain of industrial applications, especially in the rapidly growing semiconductor arena and this represents a most important segment of microscopical studies. | ||
[[File:Reflection from a bubble1.png|Reflection from a bubble1]] | [[File:Reflection from a bubble1.png|Reflection from a bubble1]] | ||
Revision as of 23:34, 7 December 2012
Reflected light microscopy is one of the method of light microscopy.
In reflected light microscope the light incident on the sample and is reflected so speculate. There is one half-mirror in which 50% of the light is reflected and 50% of the light is transmitid, with losses in intensity image, but one gains the final resolution.
Polarizing microscopy can be used with reflected and transmitted light. Reflected light is useful for study of opaque materials such as mineral oxides and sulfides, metals and silicon wafers and requires stress-free objectives that have not been corrected for viewing through a coverslip.
The reflected light microscope was created because it is not possible to observe metals with a transmitted light microscope, because the electrons of the conductive layer of metals interact strongly with photons, making these samples too little transparent.
Reflected light microscopy is often referred to as incident light, dpi-illumination, or metallurgical microscopy, and is the method of choice for fluorescence and for imaging specimens that remain opaque even when ground to a thickness of 30 micrometers. much like the fluorescense microscope, in reflected bright field microscopy the sample is illuminated from above through the objective.
Reflected light microscopy is frequently the domain of industrial applications, especially in the rapidly growing semiconductor arena and this represents a most important segment of microscopical studies.
