Recombinant DNA: Difference between revisions
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* Recombinant DNA is an '''artificially produced''' DNA. | * Recombinant DNA is an '''artificially produced''' DNA. | ||
* It is usually formed by more DNA sources from the different donor. | * It is usually formed by more DNA sources from the different donor. | ||
* The '''sticky ends''' can put together the individual strands of the DNA. | * The '''sticky ends''' can put together the individual strands of the DNA. | ||
* The ''[[restriction endonucleases]]'' cut the DNA out and ''[[DNA ligase]]'' incorporates it. | * The ''[[restriction endonucleases]]'' cut the DNA out and ''[[DNA ligase]]'' incorporates it. | ||
* For the [[recombination]] we need many and many copies of the DNA. That is the reason for using the '''[[PCR]]''' | * For the [[recombination]] we need many and many copies of the DNA. That is the reason for using the '''[[PCR]]''' – ''polymerase chain reaction''. | ||
* To incorporate the sample od the DNA into the host cell it is necessary to use the '''[[vector]]'''. | * To incorporate the sample od the DNA into the host cell it is necessary to use the '''[[vector]]'''. | ||
* After [[replication]] in the host cell | * After [[replication]] in the host cell – on the issue culture – we can work with '''the exact part of the DNA'''. | ||
* The recombinant DNA is much used in the [[Gene Manipulation|gene manipulation]]. Thanks to it we are able to produce some important human hormones and enzymes. | * The recombinant DNA is much used in the [[Gene Manipulation|gene manipulation]]. Thanks to it we are able to produce some important human hormones and enzymes. | ||
* The clones od the DNA samples can be also used for the forming of the [[Genetic Mapping|gene maps]]. | * The clones od the DNA samples can be also used for the forming of the [[Genetic Mapping|gene maps]]. | ||
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== The Process of the DNA Recombination == | == The Process of the DNA Recombination == | ||
# to isolate the exact part of the DNA from the organism | # to isolate the exact part of the DNA from the organism | ||
# to put the DNA into the DNA [[ | # to put the DNA into the DNA [[vector]] (E.coli, bacteria, yeast) | ||
# to transfer the vector by into the host | # to transfer the vector by into the host | ||
# to find out the compati '''sticky ends''' of two DNA strands | # to find out the compati '''sticky ends''' of two DNA strands | ||
# to put it together by [[DNA ligase]] | # to put it together by [[DNA ligase]] | ||
# to clone the new DNA by [[Polymerase Chain Reaction|PCR]] | # to clone the new DNA by [[Polymerase Chain Reaction|PCR]] – polymerase chain reaction | ||
# to get many copies of the recombinant DNA | # to get many copies of the recombinant DNA | ||
[[File:Recombinant-DNA.jpg|thumb|left|350px|Recombinant DNA]] | |||
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* [[DNA ligase]] | * [[DNA ligase]] | ||
* [[Gene Manipulation]] | * [[Gene Manipulation]] | ||
=== Sources=== | === Sources === | ||
* [http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html Recombinant DNA] | * [http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html Recombinant DNA] | ||
=== Bibliography === | === Bibliography === | ||
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| isbn = 80-902906-2-0 | | isbn = 80-902906-2-0 | ||
}} | }} | ||
* {{Cite | |||
| type = book | |||
| surname1 = MURRAY | |||
| others = yes | |||
| title = Harperova biochemie | |||
| edition = 4.vydání | |||
| year = 2002 | |||
| isbn = 80-7319-013-3 | |||
}} | |||
</noinclude> | </noinclude> | ||
[[Category: Biology]] | [[Category:Biology]] | ||
[[Category: Genetics]] | [[Category:Genetics]] | ||
Latest revision as of 18:10, 8 December 2014
- Recombinant DNA is an artificially produced DNA.
- It is usually formed by more DNA sources from the different donor.
- The sticky ends can put together the individual strands of the DNA.
- The restriction endonucleases cut the DNA out and DNA ligase incorporates it.
- For the recombination we need many and many copies of the DNA. That is the reason for using the PCR – polymerase chain reaction.
- To incorporate the sample od the DNA into the host cell it is necessary to use the vector.
- After replication in the host cell – on the issue culture – we can work with the exact part of the DNA.
- The recombinant DNA is much used in the gene manipulation. Thanks to it we are able to produce some important human hormones and enzymes.
- The clones od the DNA samples can be also used for the forming of the gene maps.
The Process of the DNA Recombination[edit | edit source]
- to isolate the exact part of the DNA from the organism
- to put the DNA into the DNA vector (E.coli, bacteria, yeast)
- to transfer the vector by into the host
- to find out the compati sticky ends of two DNA strands
- to put it together by DNA ligase
- to clone the new DNA by PCR – polymerase chain reaction
- to get many copies of the recombinant DNA
Links[edit | edit source]
Related articles[edit | edit source]
Sources[edit | edit source]
Bibliography[edit | edit source]
- ALBERTS,, et al. Základy buněčné biologie. 2.vydání edition. 2007. ISBN 80-902906-2-0.
- MURRAY,, et al. Harperova biochemie. 4.vydání edition. 2002. ISBN 80-7319-013-3.
