Mechanism of poisoning

Poison is a substance that, when administered in small amounts, causes disease changes or death. Due to its chemical properties. Poisons act in the body through various mechanisms:

• Etching

This term means local denaturation of tissue components by caustics, ie strong acids with pK <2, eg H 2 SO 4 , HCl or HNO 3 , or strong bases (lyes) with pK> 11.5, eg NaOH, KOH, NH 4 OH.

Iron

• Covalent nonspecific interactions with biomolecules (proteins, nucleic acids and polysaccharides).

Highly reactive aldehydes are examples of these poisons. The aldehyde group -CHO reacts readily with the amino group -NH 2 or the sulfhydryl group -SH, which is abundant in proteins. An example is methanal (formaldehyde) HCHO, its aqueous saturated solution is known as formalin.

• Disturbances of acid-base balance.

Some poisons disturb the acid-base balance of the organism. Ethylene glycol is oxidizes by alcohol dehydrogenase to glycolic, glyoxal and oxalic acids, which cause metabolic acidosis. Salicylates stimulate the respiratory center. The resulting hyperventilation leads to respiratory alkalosis. After entering the cells, they disrupt oxidative phosphorylation in the mitochondria, reduce ATP production, block citrate cycle enzymes and stimulate anaerobic glycolysis. The result is overproduction and accumulation of acidic metabolites, mainly lactate, pyruvate and acetoacetate, and thus develops metabolic acidosis.

Iron

• Effect on membranes

Ethanol, detergents and hydrocarbons change the fluidity of the membranes, which is reflected in the function of the membrane components. Changes in membrane microviscosity change the conformation of membrane channels, receptors and enzymes, and thus disrupt their functions (membrane transport, information transfer, membrane potential). Membrane fluidity is a control mechanism for heavy metal absorption.

Iron

• Interaction with oxygen transfer in the body

1. The binding of poison to hemoglobin
2. Oxidation of hemoglobin to methemoglobin Iron
3. Cytochrome oxidase inhibition Iron

• Enzyme inhibition

1. Non-specific interactions, binding to the sulfhydryl group -SH.
2. Binding of the poison to the active center of the enzyme.

• Interactions with specific receptors and impairment of cell signaling or membrane channel function

Sodium channel, nicotine and muscarinic receptors, psychotropic drugs, nddictive substancesávykové látky.