Polymerase chain reaction, application of PCR in clinical diagnostics, RT-PCR and use of this technique.: Difference between revisions
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== Mechanisms of PCR == | == Mechanisms of PCR == | ||
Three main steps of PCR: | |||
* DNA is '''denatured''' at a temperature of 90 degrees celsius. The double stranded helical DNA is seperated into two separated strands | |||
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Revision as of 20:40, 2 April 2025
Polymerase Chain reaction is method of amplifying specific sequences of DNA. It is a method that popular used in the field of medicine due to sensitivity, specificity and how quickly it yields result.
Mechanisms of PCR
Three main steps of PCR:
- DNA is denatured at a temperature of 90 degrees celsius. The double stranded helical DNA is seperated into two separated strands
