Polymerase chain reaction, application of PCR in clinical diagnostics, RT-PCR and use of this technique.: Difference between revisions
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* DNA is '''denatured''' at a temperature of 90 degrees celsius. The double stranded helical DNA is seperated into two separated strands | * DNA is '''denatured''' at a temperature of 90 degrees celsius. The double stranded helical DNA is seperated into two separated strands | ||
* | *To the sample of DNA separated strands of DNA | ||
== References == | |||
Rodwell, V.W. ''et al.'' (2018) ''Harper’s Illustrated Biochemistry 31''. 31st edn. New York: McGraw-Hill Medical. Pg 1071 - 1074 | |||
''Polymerase chain reaction (PCR)'' (n.d) ''Khan Academy''. Available at: <nowiki>https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/polymerase-chain-reaction-pcr#:~:text=PCR%20is%20used%20in%20many%20research%20labs%2C%20and%20it%20also,the%20case%20of%20prenatal%20testing</nowiki>). (Accessed: 02 April 2025). | |||
Khehra, N., Padda, I.S. and Swift., C.J. (2023) ''Polymerase chain reaction (PCR)'', ''StatPearls [Internet].'' Available at: <nowiki>https://www.ncbi.nlm.nih.gov/books/NBK589663/</nowiki> (Accessed: 02 April 2025). | |||
''PCR technique and its applications in biology and medicine'' (2023) ''MyBioSource Learning Center''. Available at: <nowiki>https://www.mybiosource.com/learn/pcr-technique-and-its-applications-in-biology-and-medicine/</nowiki> (Accessed: 02 April 2025). | |||
Revision as of 20:43, 2 April 2025
Polymerase Chain reaction is method of amplifying specific sequences of DNA. It is a method that popular used in the field of medicine due to sensitivity, specificity and how quickly it yields result.
Mechanisms of PCR
Three main steps of PCR:
- DNA is denatured at a temperature of 90 degrees celsius. The double stranded helical DNA is seperated into two separated strands
- To the sample of DNA separated strands of DNA
References
Rodwell, V.W. et al. (2018) Harper’s Illustrated Biochemistry 31. 31st edn. New York: McGraw-Hill Medical. Pg 1071 - 1074
Polymerase chain reaction (PCR) (n.d) Khan Academy. Available at: https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/a/polymerase-chain-reaction-pcr#:~:text=PCR%20is%20used%20in%20many%20research%20labs%2C%20and%20it%20also,the%20case%20of%20prenatal%20testing). (Accessed: 02 April 2025).
Khehra, N., Padda, I.S. and Swift., C.J. (2023) Polymerase chain reaction (PCR), StatPearls [Internet]. Available at: https://www.ncbi.nlm.nih.gov/books/NBK589663/ (Accessed: 02 April 2025).
PCR technique and its applications in biology and medicine (2023) MyBioSource Learning Center. Available at: https://www.mybiosource.com/learn/pcr-technique-and-its-applications-in-biology-and-medicine/ (Accessed: 02 April 2025).
