Polymerase chain reaction, application of PCR in clinical diagnostics, RT-PCR and use of this technique.
From WikiLectures
Polymerase Chain reaction is method of amplifying specific sequences of DNA. It is a method that popular used in the field of medicine due to sensitivity, specificity and how quickly it yields result.
Mechanisms of PCR
Three main steps of PCR:
- DNA is denatured at a temperature of 90 degrees celsius. The double stranded helical DNA is seperated into two separated strands
