Urinary ketone bodies/determination
(Redirected from Ketolátky v moči/stanovení)
The detection of ketone bodies is based on the reactions of acetoacetic acid and acetone with sodium nitroprusside in an alkaline medium, which forms a red-violet-colored complex . This principle is used by Legal's and Lestradet's exams , as well as diagnostic strips. Β-hydroxybutyric acid (ie the most abundant ketone substance) does not provide a reaction and therefore a negative result does not completely rule out ketoacidosis.
Compounds with free sulfhydryl groups (eg the antihypertensive captopril or uroprotectant used in some mesna chemotherapeutic regimens ) provide false positivity for urinary ketone bodies . Quite often, bacterial products in urinary tract infections also provide a similar response.
False negatives , apart from the already mentioned insensitivity of the β-hydroxybutyric acid tests, are not significant.
Odkazy[edit | edit source]
Reference[edit | edit source]
Kategorie:Vložené články Kategorie:Biochemie Kategorie:Klinická biochemie