Fetal membranes (SFLT)
Fetal membranes – overview (HE)[edit | edit source]
Description: Fetal envelopes include (from fetus to uterus): Aep – amniotic epithelium, EEM – extraembryonic mesoderm, T – trophoblast, D – decidua capsularis, AD – amniotic cavity, filled with amniotic fluid in vivo.
Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!
Fetal membranes – a closer look (HE)[edit | edit source]
Description: Fetal envelopes include (from fetus to uterus): Aep – amniotic epithelium, EEM – extraembryonic mesoderm, T – trophoblast, D – decidua capsularis, AD – amniotic cavity, filled with amniotic fluid in vivo.
Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!
Fetal membranes – amnion and extraembryonic mesoderm (HE)[edit | edit source]
Description: Aep – amniotic epithelium – one layer of cubic cells, washed in vivo by amniotic fluid (in the amniotic cavity – AD), it lies on a clearly visible basement membrane, under this epithelium is the extraembryonic mesoderm (EEM).
Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!
Extraembryonic mesoderm, trofoblast and decidua (HE)[edit | edit source]
Description: EEM – extraembryonic mesoderm, VV – vascular layer of extraembryonic mesoderm (well-perfused layer adjacent to trophoblast), T – trophoblast, D – decidua capsularis. Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!
Fetal membranes – overview (proof of pan-cytokeratin - immunohistochemistry)[edit | edit source]
Description: Detection of keratin using antibodies. Keratin is present in cells of epithelial origin.
Thus, amniotic epithelium (Aep) and trophoblast (T) were shown. The fibrous layers – extraembryonic mesoderm (EEM) and decidua (D) – are therefore not highlighted. AD – amniotic cavity, VV – vascular layer of extraembryonic mesoderm (well-perfused layer adjacent to the trophoblast).
Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!
Fetal membranes – amnion (proof of pan-cytokeratin - immunohistochemistry)[edit | edit source]
Description: Detection of keratin using antibodies. Keratin is present in cells of epithelial origin.
Thus, the amniotic epithelium (Aep) and not the extraembryonic mesoderm (EEM, ligament) was highlighted. AD – amniotic cavity
Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!
