Haemophilus ducreyi

Haemophilus ducreyi is a gram-negative, facultatively anaerobic rod in chains. It is the cause of a soft ulcer (ulcus molle) on the genitals. It is a strictly human pathogen.

Cultivation

Cultivation of hemophilia is generally demanding, requiring soils enriched with growth factors. H. ducreyi grows in the presence of factor X (hemin). Colonies are visible after 2-9 days.

Diagnostics

Microscopy of material from the ulcer or aspirate from the affected lymph nodes shows G− rods in chains inside and outside the polymorphonuclear cells. The painting is often described as a "school of fish". Rich soils (eg chocolate agar) and CO2 atmosphere are used in the cultivation, a yolk bag of chicken embryos can also be used. In an experiment on an animal (rabbit), ulceration occurs after injection of a pure culture.

An indirect evidence is the so-called Ducrey test, in which inactivated hemophiles are applied subcutaneously. A positive test will show a local inflammatory reaction.

Treatment

Macrolides (azithromycin) and cephalosporins (ceftriaxone) are used for therapy.

Links

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References

1. NGUYEN, Andrew. Chancroid in Emergency Medicine [online]. ©2016. Poslední revize 2016-07-26, [cit. 2017-01-20]. <https://emedicine.medscape.com/article/781520-overview>.
2. ↑ GOERING, Richard V a Hazel M DOCKRELL. Mimsova lékařská mikrobiologie. 5. vydání. Praha : Triton, 2016. 568 s. s. 264. ISBN 978-80-7387-928-0.
3. ↑ Skočit nahoru k: a b VOTAVA, Miroslav, et al. Lékařská mikrobiologie speciální. 1. vydání. Brno : Neptun, 2003. 495 s. s. 82. ISBN 80-902896-6-5.
4. ↑ JULÁK, Jaroslav. Úvod do lékařské bakteriologie. 1. vydání. Praha : Karolinum, 2006. 404 s. s. 249. ISBN 8024612704.

Source

• ws:Haemophilus ducreyi