Identification of restriction fragments
From WikiLectures
Identification of fragments is carried out using so-called samples. A probe is a short natural or artificial DNA of known sequence, labeled with radioactive or fluorescent ethidium bromide. Electrophorogram of the fragments (the so-called "coating") is transferred to a nitrocellulose membrane (blotting), the DNA is denatured and poured over with a sample solution. The monitored fragment associates with the probe (base pairing) and after washing off the "coating" is autoradiographically or fluorescence identified among hundreds of other fragments.
Sequencing methods are among genetic engineering methods. It serves to clarify the structure of the entire fragment (the sample usually represents only a small section of the fragment chain).
Links[edit | edit source]
Related Articles[edit | edit source]
- Genetic Engineering Biochemistry
- DNA Cleavage
- Separation of DNA fragments by electrophoresis
- Artificial DNA Synthesis
- Multiplication and expression of an isolated gene in a host cell
- Restriction fragment length polymorphism
Source[edit | edit source]
- PINCH, Stanislav. Concise Biochemistry : storage and expression of genetic information. 1. edition. Medprint, 1998. ISBN 80-902036-2-0.
References[edit | edit source]
- PINCH, Stanislav. Concise Biochemistry : storage and expression of genetic information. 1. edition. Medprint, 1998. ISBN 80-902036-2-0.
