Lambert-Beer law

The Lambert-Beer law determines absorbance, a quantity characterizing the absorption of electromagnetic radiation in a substance.

Absorbance depends on the thickness of the $d$ layer, where the radiation is attenuated, and on the absorption coefficient $\alpha$ (attenuation), which depends on intensity.

$A=\alpha \cdot d$

We substitute Beer's law into this formula, where $\varepsilon$ is the molar absorption coefficient and $C$ is the molar concentration of the solution:

$\alpha =\varepsilon \cdot C$

We thus obtain the Lambert-Beer law:

$A=\epsilon \cdot C\cdot d\!$

Links

Related Articles

• Photometry
• Beer's Law
• Absorbance
• Light Absorption

External links

• ws:Lambert-Beerův zákon
• Wikipedia. Light Absorption [online]. [cit. 2012-31-12]. <https://cs.wikipedia.org/wiki/Absorption_sv%C4%9Btla>.

Source

• RETURNED, Leoš – ROSINA, Joseph, et al. Medical Biophysics. 2010. edition. 2005. 524 pp. ISBN 978-80-247-1152-2.