Nitrotetrazolium blue

Reduction of nitrotetrazolium blue (NBT) to colored formazan

Nitroblue tetrazolium (NBT) is used to detect reducing substances. Reduction of tetrazolium rings in NBT leads to their opening, forming formazan^[1]. In doing so, the absorption spectrum of the substance changes and the originally light yellow color changes to dark blue. The reaction is suitable for spectrophotometric determination.

NBT is a fairly widely used reagent. It is used, for example, to determine glycated proteins, a fairly typical application is also the determination of superoxide production in various systems.

Links

References

↑ BIELSKI, Benon HJ – SHIUE, Grace G – BAJUK, Stanley. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. Journal of physical chemistry [online]. 1980, vol. 84, no. 8, p. 830-833, Available from <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639. DOI: 10.1021/j100445a006.

[1] BIELSKI, Benon HJ – SHIUE, Grace G – BAJUK, Stanley. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. Journal of physical chemistry [online]. 1980, vol. 84, no. 8, p. 830-833, Available from <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639. DOI: 10.1021/j100445a006.

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