Soil inoculation

Inoculation on a petri dish

Method of application in a petri dish

• we sterilize the loop in the non-luminous flame of the torch
• after the loop has cooled, we take one colony from the pre-prepared culture and transfer it to agar in a petri dish as follows:

1. apply the sample to the edge of the petri dish, carefully spread in the range of about 3x1 cm
2. we sterilize the loop
3. at a certain angle we intersect the previous line with two strokes and add two more strokes that no longer intersect with it
4. we sterilize the loop and apply the previous point again (approx. 2x)

Inoculation on slant agar

Method of application in a test tube

If the sample is applied to slant agar (in a test tube), the sample is also applied with a sterilized loop from the bottom up in a wavy motion.

Broth inoculation

In liquid broth, apply the colony to the inner wall of the tube just above the level of the broth and then tilt the tube to wash the sample from the wall and mix with the broth.

Inoculation of anaerobic bacteria

Anaerobic bacteria need an oxygen-free environment for their growth. Apply the colonies to the agar in the standard way and place the closed petri dishes in an anaerostat, which is a special tightly closable container. Here, an anaerobic environment is created using special chemicals that, when water is added, drain the oxygen in the air.

Link

related articles

• Cultivation soil
• Blood culture

Source

• RYŠKOVÁ, Olga. Návody k praktickým cvičením z lékařské mikrobiologie. 1. edition. Praha : Karolinum, 1997. ISBN 80-7184-307-5.

• ws:Očkování na půdy