Portal:Questions for final examination in biochemistry
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I. PHYSICAL, BIOANORGANIC AND BIOORGANIC CHEMISTRY[edit | edit source]
- Physical and chemical properties of water, its importance in human organism
- Solubility of compounds, analytical and colloidal dispersions, diffusion, osmosis, dialysis, oncotic pressure, biochemical examples
- Energetics of chemical reactions, enthalpy, entropy, Gibb?s energy, metabolic applications
- Kinetics of reversible and consecutive reactions, chemical equilibrium, equilibrium law
- Kinetics of chemical reactions, application to enzymology
- Catalysts and biocatalysts, importance for biochemistry
- Electrolytic dissociation, dissociation constant, strong and weak electrolytes, biochemical examples
- Brönsted?s theory of acids and bases, equilibrium in protolytic reaction biochemical examples
- Ionic product of water, pH and its importance for medicine
- Ampholytes, isoelectric point, biochemical examples
- Buffer solution, pH calculation, importance in human organism
- Oxidation and reduction, biochemical examples
- Redox potential, dependency on the concentration of the reactants and pH, biochemical examples
- Solubility product, biochemical examples
- Complex formation, importance for biochemistry and medicine
- Chemical properties of basic biogenic elements. Reactive forms of oxygen
- Biologically and toxicologically important elements of the main groups of the periodic table
- Biologically and toxicologically important nontransition elements. Nitrogen inorganic compounds of biological importance
- Trace elements ? biological and metabolic importance
- Structure of organic compounds, isomerism, examples in metabolic pathways
- Relationship between structures and properties of organic compounds, biochemical examples
- Medically and toxicologically important halogeno and nitroderivatives of hydrocarbons
- Medically important sulphur-containing derivatives of hydrocarbons
- Amines, importance in biochemistry
- Alcohols, phenols, aldehydes, ketones, biochemical examples
- Carboxylic acids, biochemical examples
- Esters of carboxylic and inorganic acids, biochemical examples
- Functional and substituted derivatives of carboxylic acids, biochemical examples
- Importance of nitrogen, oxygen and sulphur-containing heterocycles
- Proteogenic amino acids, division, structures, reactions, peptides, biological importance
- Proteins - structure, properties and functions. Conformation of proteins, importance for their biological function
- Saccharides, classification, structure, stereochemistry, biological importance
- Reactions of saccharides, disaccharides, N- and O- glycosidic bond, examples
- Homopolysaccharides and heteropolysaccharides
- Proteoglycanes, glycoproteins, structure, properties
- Lipids, definition, classification, structure, properties, functions in human organism
- Phosphatidic acid and its derivatives
- Phospho- anf glycosphingolipids, structure properties, function in human organism
- Sterols, classification, structure, properties, functions in human organism
- Bile acids and steroid hormones ? structure, properties and function in human organism
II. METABOLISM[edit | edit source]
- Structure of enzymes (coenzymes, oligomeric structure). Multiple forms of enzymes and isoenzymes. Medical application of enzymology
- Enzymatic activity, influence of physical and chemical conditions, regulation (expression, allosteric effects, covalent modification)
- Respiratory chain Oxidative phosphorylation
- Energy-rich compounds, substrate level phosphorylation, drive of endergonic reactions
- Citric acid cycle, amphibolic character of the citric acid cycle, regulation
- Cell membrane - structure, assembly, function, transport across membranes
- Common mechanisms of amino acids conversion, deamination, transamination, nitrogen balance
- Formation and detoxification of ammonia, urea cycle and its regulation, hyperammonaemia
- Catabolism of the carbon skeleton of amino acids, integration of amino acids into metabolic pathways
- Amino acids of pyruvate, oxalacetate and 2-oxoglutarat family, their catabolism
- Amino acids of succinyl CoA family, their catabolism
- Metabolism of branched amino acids
- Metabolism of phenylalanine and tyrosine, defects
- Metabolism of tryptophane, major and minor pathways, defects in its resorption
- Metabolism of sulphur-containing amino acids
- Conversion of amino acids into special products (formation, conversion and importance of creatine, formation, conversion and importance of S-adenosylmethionine)
- Biosynthesis, biodegradation and function of the most important biogenic amines and catecholamines
- Glycolysis, regulation
- Gluconeogenesis, regulation
- Degradation and synthesis of glycogen, regulation, defects
- Pentose phosphate pathways, regulation
- Metabolism of galactose and fructose, defects
- Metabolism of glucuronic acid and its metabolic importance
- Biosynthesis "de novo" of saturated fatty acids, regulation. Origin of acetyl CoA for biosynthesis of palmitate
- Palmitate as substrate for synthesis of unsaturated fatty acids and long-chain fatty acids
- Formation of ketone bodies from acetyl-CoA, metabolic causes and importance
- Carnitine system, oxidation of fatty acids, energy balance
- Biosynthesis, conversion and transport of triacylglycerols
- Structure, metabolism and function of phosphatide acid and phospholipids
- Biosynthesis of sphingosine, sphingolipids, structure, properties and function in organism
- Synthesis and degradation of eicosanoids, cyclooxygenase and lipooxygenase pathways
- Biosynthesis of cholesterol and its regulation, role of HMG-CoA reductase. Transport of the endogenic and exogenic cholesterol
- Conversion and excretion of cholesterol, bile acids
- Biosynthesis, degradation and function of steroid hormones
- Transport of lipids, role of lipoproteins, structure of lipoprotein particles. Formation, conversion, and role of chylomicrons, VLDL, LDL and HDL lipoproteins
- Biosynthesis of hem, defects of synthesis, porphyrias
- Degradation of tetrapyrroles, hyperbilirubinaemia, jaundices
- Pyrimidine nucleotides, metabolism, regulation, inhibitors, defects
- Purine nucleotides, metabolism, regulation, inhibitors, defects
- Biosynthesis of deoxyribonucleotides and its regulation, inhibitors
III. FUNDAMENTALS OF ORGAN AND FUNCTIONAL BIOCHEMISTRY.[edit | edit source]
- Regulation and coordination of carbohydrate and lipid metabolism
- Glycaemia and its regulation. Glucose tolerance test. Glycosylated haemoglobin
- Metabolic interrelation between glucose and other nutrients; Cori cycle
- Metabolism of fat tissue
- Regulation of haeme biosynthesis; differences between the hepatocyte and erythroid cell; iron metabolism
- Regulation of metabolism at the neurohumoral level, integration systems of the organism
- Hormonal regulation of water and mineral metabolism
- Hormonal regulation of bone metabolism
- The integration of metabolism and the provision of tissue fuel
- Biochemical processes in the stomach; gastric juice; gastric mucins, acid-resistant lipases
- Intestinal digestion; role of the pancreas in the digestion
- Biochemical functions of hepatocytes and liver; assessment of liver function and damage
- Biotransformation of endogenous and exogenous compounds; phases of the biotransformation. Toxic and carcinogenic compounds in the environment
- Buffer systems of the organism
- Bicarbonate system, its significance in maintaining the acido-base balance
- The role of haemoglobin, proteins and phosphates in maintaining the acido-base balance
- Erythrocyte metabolism
- Haemocoagulation
- Urine, properties and its physiological and pathological constituents
- Collagen family ? structure, properties, metabolism, function
- Adhesive glycoproteins of the extracellular matrix
- Biochemistry of the cartilage and bone
- Biochemistry of the skin. Biochemical basis of touch
- Contractile apparatus, the control of smooth and skeletal muscle contraction
- Biochemistry of vision - Wald cycle, transducin cycle, the role of cGMP-gated ion channels
- Biochemistry of hearing
- Biochemistry of olfaction
- Biochemistry of taste
- Biochemical events in the synapsis; neurotransmitters
- Receptors ? structure and function
- Steroid hormones and their functions in regulatory processes
- Peptide hormones and their functions in regulatory processes
- Thyroid hormones and their functions in regulatory processes
- Prostaglandins, thromboxanes, leukotrienes, lipoxins. Hydroxyepoxytriene acids
- Molecular basis of the humoral immune response
- Molecular basis of the cellular immune response
- Lipid-soluble vitamins
- Water-soluble vitamins
- Structure, constituents and properties of cell membranes
- Membrane transport
IV. BASIC CELLULAR AND MOLECULAR BIOLOGY[edit | edit source]
- Signal transduction, membrane receptors and their ligands, G-proteins
- Second messenger pathways and signal transduction
- Signalling using Ras proteins, cascade of kinases, JAK-STAT signaling pathway
- Activation of phospholipases in signal transduction
- NO signalling. Overview of signalling pathways and significance
- Intracellular receptors and their ligands, HSP, interactions of intracellular receptors with DNA
- Intercellular communication
- Structure and function of nucleic acids
- Organization of prokaryotic, eukaryotic and mitochondrial genome
- Replication of eukaryotic DNA, DNA replication apparatus and its regulation
- DNA repair
- Transcription of eukaryotic DNA, transcription apparatus and its regulation, cis- and trans-regulation elements
- mRNA, structure, synthesis, posttranscriptional modifications, RNA interference
- Regulation of gene expression at the level of transcription
- Genetic code and its properties
- Eukaryotic, prokaryotic and mitochondrial translation
- Regulation of gene expression at the level of translation, inhibitors of translation, antibiotics as translation inhibitors
- Posttranslational modification of proteins
- Protein transport and targeting, modification and sorting of proteins in Golgi apparatus
- Biosynthesis of glycoproteins, their structure and significance
- Restriction enzymes and their usage, DNA modification and construction of recombinant DNA molecules
- DNA cloning, transfer of foreign genetic information into bacterial and mammalian cells
- DNA diagnostics, techniques, RFLP
- Gene therapy, transfer of the genetic material in gene therapy
- Blotting, hybridization techniques and their application in clinical diagnostics, hybridization probes
- Methods of cell fractionation, electrophoretic analysis of nucleic acids and proteins
- Polymerase chain reaction, PCR applications in clinical diagnostics, RT-PCR and application of this technique, DNA sequencing
- Hereditary diseases and their diagnostics
- Hereditary predisposition to cancer and its diagnostics
- Types of human gene mutations, hereditary and somatic mutations
- Polymorphisms, significance and detection, minisatellite and microsatellite markers and their analysis
- Retroviruses and pararetroviruses, structure and replication
- Protooncogenes and tumor suppressor genes
- Cell cycle, cyclins and cdks complexes
- Regulation of cell cycle, the role of tumor suppressor genes
- Ubiquitination and proteasomal degradation of proteins
- Apoptosis,initiation, control and effector phase, death receptors and their ligands, caspases
- Apoptosis, intrinsic and extrinsic activation pathways, the role of mitochondria in apoptosis
- Epigenetics, DNA methalation, histone modifications, epigenetic effects in humans
- DNA recombination, the roles of homologous and nonhomologous recombination