Plasmacoagulase test

This test is used to distinguish bacteria within the Staphylococcus genus. The plasmacoagulase test demonstrates the presence of a plasmacoagulase protein with enzymatic activity that converts fibrinogen into solid fibrin.

Positive result of the plasmacoagulase test.

Principle: Coagulase-forming strains convert the liquid plasma into which they have been inoculated into a solid coagulum. It is important not to forget the possibility of false negatives, as some strains also produce the enzyme staphylokinase (also fibrinolysin), which dissolves fibrin. Therefore, checks at 2, 6, 18 and 24 hours after plasma inoculation are necessary.

Results:

• positive – Staphylococcus aureus
• negative – Staphylococcus epidermidis and other Staphylococci

Links

Related articles

• Bacitracin test
• CAMP test
• Catalase test
• Optochin test
• PYR test

Literatures

• BEDNÁŘ, M – FRAŇKOVÁ, V – SCHINDLER, J, et al. Lékařská mikrobiologie – bakteriologie, virologie, parazitologie. 1. edition. Praha : Marvil, 1996. 558 pp. ISBN 80-238-0297-6.

• ŠMÍROVÁ, Václava. Úvod do lékařské mikrobiologie. - edition. -.